Regulatory

Part:BBa_K4998021:Design

Designed by: Rafail Andreou   Group: iGEM23_thessaloniki   (2023-10-09)


trc promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For the IPTG induction, a lacI gene should also be inserted/expressed in the selected strain's genome.


Source

Nostoc oryzae TAU-MAC 2710

References

[1] Kokarakis, E. J., Rillema, R., Ducat, D. C., & Sakkos, J. K. (2022). Developing cyanobacterial quorum sensing toolkits: Toward interspecies coordination in mixed autotroph/heterotroph communities. ACS Synthetic Biology, 12(1), 265–276. https://doi.org/10.1021/acssynbio.2c00527